Journal: Materials Today Bio

Article Title: Hyaluronic acid-based hydrogel microspheres with multi-responsive properties for antibacterial therapy and bone regeneration in Staphylococcus aureus -infected skull defects

doi: 10.1016/j.mtbio.2025.101676

Figure Lengend Snippet: Effects of osteogenesis and angiogenesis analyses on BMSCs and HUCEs. (a,b) Immunofluorescence staining of OPN and OCN under different conditions. (c) Quantitative analysis of immunofluorescence staining in different groups. (d) Gross and enlarged images of ARS and ALP. (e) Assay results and analysis of ARS and ALP activity in different groups. (f–k) Real-time RT-PCR was performed to determine the gene expression of OCN, OPN, Col-1, ALP, CD31, and VEGF (n = 3). (l–o) Western blot performed to analyze protein expression of OCN, OPN, Col-1, ALP, CD31, and VEGF levels (n = 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.

Article Snippet: ALP activity and staining were conducted on day 14 using kits from Beyotime Biotech and Leagene Biotechnology, respectively, following the instructions.

Techniques: Immunofluorescence, Staining, Activity Assay, Quantitative RT-PCR, Gene Expression, Western Blot, Expressing

Journal: BMC Oral Health

Article Title: Increased bone mass but delayed mineralization: in vivo and in vitro study for zoledronate in bone regeneration

doi: 10.1186/s12903-024-04906-2

Figure Lengend Snippet: A - B , I , J ZOL upregulated the expression of BMP4 and OCN in osteoblasts at typical concentrations (* P < 0.05). C , E , K , L After seeding at a low density, ALP staining revealed that the amount of colored sediment in the culture well decreased with ZOL stimulation in a dose-dependent manner. At a higher density, the trend reversed. D , F , P ZOL decreased cell proliferation in a time- and concentration-dependent manner. G , O ZOL strongly hindered calcium nodule formation at all concentrations. H ZOL altered the cellular morphology and induced apoptosis. 1x = 0.02 µg/ml, 5x = 0.1 µg/ml, 25x = 0.5 µg/ml

Article Snippet: ALP activity staining kits (Beyotime, Shanghai, China) were used to evaluate osteogenic differentiation. (Note: osteogenic induction medium was not applied for ALP activity assessment).

Techniques: Expressing, Staining, Concentration Assay

Journal: Regenerative Biomaterials

Article Title: Surface-modified deproteinized human demineralized tooth matrix for bone regeneration: physicochemical characterization and osteoblast cell biocompatibility

doi: 10.1093/rb/rbae030

Figure Lengend Snippet: ( A ) The quantification of cell viability, ( B ) ALP activity, and ( C ) osteocalcin activity of dpDTM, A-dpDTM, and C-dpDTM. *Significant difference between the groups at P < 0.05, Ø significant difference with preceding time point at P < 0.05.

Article Snippet: Alkaline phosphatase (ALP) was measured using ALP activity staining kit (Human Diagnostics Worldwide, Magdeburg, Germany), in accordance with manufacturer’s instructions.

Techniques: Activity Assay

Journal: Scientific Reports

Article Title: Multifunctional Zn and Ag co-doped bioactive glass nanoparticles for bone therapeutic and regeneration

doi: 10.1038/s41598-023-34042-w

Figure Lengend Snippet: Osteogenesis macroscopic of treated hFOB 1.19 with particles at the concentration of 125 µg/mL ( a ) stained by Alizarin Red S staining and ( b ) stained by ALP activity staining kit following 21 days in culture. The basal and osteogenic media served as the negative and positive control, respectively.

Article Snippet: On the 21st day in culture, osteoblastic differentiation of hFOB 1.19 cells was measured via cellular ALP activity staining (Abcam) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Staining, Activity Assay, Positive Control

Journal: PLoS ONE

Article Title: Impaired Osteoblast Differentiation in Annexin A2- and -A5-Deficient Cells

doi: 10.1371/journal.pone.0107482

Figure Lengend Snippet: (A) ALP activity staining in MC3T3-E1 cells (MC3T3), Si, AnxA2 kd and AnxA5 kd cells after culture in osteogenic differentiation media for 7, 14 and 21 days, representative images from n = 3 biological replicates. (B) Quantitation of ALP staining intensity. Bars represent mean integrated signal intensity ± SEM, n = 3. + represents statistically significant difference from same genotype on day 0, p<0.05. **represented statistically significant different from Si at the same day, p<0.01. (C) OsteoImage staining for hydroxyapatite in Si, AnxA2 kd and Anx5 kd cells cultured with differentiation media for 5 weeks. Each bar represents OsteoImage fluorescence normalized to PI ± SEM, n = 3. ***represents statistically significant difference from maintenance media within the same genotype, ***p<0.001; + represents statistically different from Si in same media composition, p<0.01.

Article Snippet: Cells were seeded in 35-mm plates and grown for 7, 14 and 21 days in osteogenic differentiation medium and stained with an ALP activity staining kit (Sigma) according to manufacturer’s instructions.

Techniques: Activity Assay, Staining, Quantitation Assay, Cell Culture, Fluorescence